FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14770143
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTAT838190.567489427827476TruSeq Adapter, Index 15 (97% over 36bp)
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC228120.1544467105023966No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG164130.11112282392932823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA329300.043.051951
AGAGCAC361150.038.9493948
GAGCACA362950.038.9346129
TCGGAAG374700.038.0080873
ATCGGAA397100.035.7914922
CGGAAGA446250.032.134644
AAGAGCA477250.029.9568147
GGAAGAG500150.028.6715645
GAAGAGC498000.028.6363376
AGTCACA211300.013.34145425-29
CTCGAAT211900.013.323997535-39
TCGAATC215250.013.17683135-39
TCACACG210350.013.15525830-34
CTCGTAT206600.013.06648840-44
GTCACAC213800.013.0238325-29
CGAATCT212850.012.8113335-39
CAGTCAC224000.012.70073725-29
TCTCGTA230850.011.89971140-44
CGTATGC244150.011.77047745-49
TCGTATG236250.011.49980245-49
Produced by FastQC (version 0.11.5)