FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14770143
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGTGGTGTGTAGATCT852840.5774080860286863Illumina Single End PCR Primer 1 (97% over 35bp)
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG263240.17822440852468388No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA180780.12239556516142058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA335900.041.9044461
TCGGAAG378800.037.094723
GAGCGTC381650.036.3645489
AGAGCGT388400.035.8059738
ATCGGAA400650.035.3561552
AAGAGCG422950.033.0855037
CGGAAGA449800.031.3514234
GGAAGAG497600.028.4113335
GAAGAGC497000.028.3007976
TAGATCT198250.014.37032640-44
TACAGTG196700.014.22064330-34
AGTGTAC199000.014.04955325-29
GTGTGTA198000.013.98131835-39
GTACAGT207950.013.59669530-34
TGTGTAG203600.013.56141140-44
ACAGTGG208250.013.52871730-34
CGTATCA220550.013.2308660-64
GTAGATC221300.012.97763440-44
GGTGTGT218650.012.94398735-39
CAGTGGT218200.012.918430-34
Produced by FastQC (version 0.11.5)