FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11468665
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGC19000.029.5216583
ACTAGCA23150.025.4665324
CTAGCAA24800.023.7721865
TTACTAG27700.019.2110752
CTACACA33400.014.8528424
TAGCAAC42850.013.9262516
ACACATA48800.010.3130046
TACACAT50150.09.8920235
AGCAACC64650.09.5640247
GCAACCC68800.09.0916268
TCTACAC57650.08.8564323
CAACCCC79250.08.255689
ATTACTA73250.08.129811
CACATAC64400.07.703257
ACATACT65350.07.70128448
TTCTACA94650.06.0781182
CTTCTAC94650.05.90805241
CATACTT89100.05.8905519
Produced by FastQC (version 0.11.5)