FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14603480
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT73250.027.5223148
GCCCTTA79900.027.1240354
GTAGCCC84200.025.5739481
CACTATA80100.024.6290769
TAGCCCT88600.024.5449032
AGCCCTT92750.023.3661483
AACACTA86800.023.2259187
CAAACAC100250.020.3971045
AAACACT115600.018.0000866
CCCTTAT127800.017.3521945
ACCAAAC116750.017.3294183
CCAAACA131400.015.5069224
CCTTATT167800.013.8594236
TATTTAG194350.012.3364389
CCCCACC76350.09.1495841
CTTATTT284500.08.604617
CCCCTCA50250.08.53953785-89
GGCCCCA50300.08.531054545-49
TACACTC54400.08.31161115-119
CCCACCG81000.08.0899252
Produced by FastQC (version 0.11.5)