FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14998051
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACC95600.08.0643111
AATCACG92800.07.05918938
CAATCAC102050.06.4898087
CCACCAA123100.06.0863683
CCCACCA117450.05.94975042
CACCAAT113400.05.84463934
GGTAACT102900.05.6369941
CCAATCA121550.05.6263366
ATCACGC125800.05.60790979
GGTAAGA124550.05.6003571
ACCAATC125200.05.40481145
GTTCACT70000.05.34764247
CCCCCTA20859.799247E-45.28227571
GGTAAAA183300.05.12723641
Produced by FastQC (version 0.11.5)