FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16552763
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTAT193040.1166210136640028TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA259650.012.254539
AGAGCAC262550.012.0643348
GATCGGA328750.09.8823291
TCGGAAG362050.09.0369093
AAGAGCA362600.08.9538567
GAAGAGC392450.08.4192366
ATCGGAA403750.08.2642052
GGAAGAG462100.07.1346695
CGGAAGA500700.06.60274934
CTCGTAT109900.06.56327640-44
AGTCACA111800.06.309989525-29
CTAGATC18550.06.2866516140-144
TCTCGTA139650.05.278489640-44
TCGTATG130850.05.14982745-49
CAGTCAC136450.05.09621725-29
GTCACAA144900.05.057358325-29
TAGATCG87700.05.023427140-144
CCAGTCA143950.05.000721525-29
Produced by FastQC (version 0.11.5)