FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16174143
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACA68550.014.4907275
AAGGTAC76000.013.5623052
TACACAA75400.013.2697286
AGGTACA89150.011.14240553
GGTACAC93650.010.7607234
ACACAAA120700.08.2298197
AAAGGTA140500.08.153271
CTAACAC29550.07.112317655-59
CACAAAG173300.06.27184878
ACAAAGC174550.06.2683679
GCTAACA40950.06.011107450-54
TGCTAAC40400.05.558473650-54
Produced by FastQC (version 0.11.5)