FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505628
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA74600.032.81249
GCCACTC78550.031.2540328
ACGTTAA53600.024.7135494
AGCCACT128600.019.3694487
TACGTTA68050.019.2584533
AAGCCAC128450.019.0553846
GTGAGTA47200.018.6121252
GTTACGT70150.018.341871
CGTTAAA76500.017.3152715
AGTGAGT54350.016.9864331
ACAAAGC187600.013.2039463
TAACCCC72850.012.5508189
TACAAAG211500.012.3246992
GGCCTAA43450.011.53179975-79
TAGCATG71700.010.6435048
TTACGTT131550.010.5096582
ATACAAA262450.010.3868221
AAAGCCA254000.010.1466395
GTTAAAT143500.09.5819616
GGGGCCC54900.09.546427105-109
Produced by FastQC (version 0.11.5)