FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13587716
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGTGGTGTGTAGATCT340170.2503511259729008Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA232450.025.2385851
TCGGAAG234050.024.7266523
ATCGGAA260600.022.5366252
AGAGCGT265200.022.0668168
GAGCGTC296700.019.9181189
CGGAAGA306300.019.1526344
AAGAGCG313450.018.8307087
GGAAGAG348500.017.0384125
GAAGAGC378450.015.5759056
GTGTGTA89000.013.47660735-39
TACAGTG94200.012.3804130-34
ACAGTGG98100.012.04966730-34
AGTGTAC102150.011.715569525-29
TGTGTAG101500.011.53185740-44
CAGTGGT108550.011.07535330-34
GGTGTGT111200.010.96741335-39
GTACAGT111500.010.52406930-34
GAGTGTA118050.010.14981325-29
GTGTACA120250.09.95214425-29
TAGATCT124000.09.92702640-44
Produced by FastQC (version 0.11.5)