FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16340359
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC21353.3105607E-107.75480277
GTTAGAC27851.2654709E-86.2034346
TACACGG92500.05.58869741
TAGTTAG34704.038884E-85.3948794
TACATGC79800.05.3832321
TACATCC126950.05.2191851
TACATAT133100.05.19684271
TACTTTG150050.05.1920851
TACTCGC74600.05.17286351
TACACAA96150.05.1493641
TACTCGT81100.05.1173841
Produced by FastQC (version 0.11.5)