FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15718019
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT81800.022.442438
CACTATA85250.021.4497539
AACACTA96250.019.2227447
CAAACAC99250.018.9325085
AAACACT109050.017.0984636
CCAAACA121750.015.3154224
ACCAAAC133250.013.7776553
GCCACTC49250.013.0096848
CCACTCA50800.012.4710199
TAGCCCT72750.09.9987452
GCCCTTA84650.09.1853344
GTAGCCC81250.08.8864661
AAGCCAC86550.07.5693286
AGCCACT90650.07.30639277
AGCCCTT107900.07.20617253
CCCCTCA57550.06.830216485-89
TACCAAA327400.06.6433232
ATACCAA355350.06.40347241
GGCCCCA64750.06.271237445-49
TATTTAG145550.06.18273749
Produced by FastQC (version 0.11.5)