FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15036560
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAATC93300.011.4969566
TACCAAT104500.010.4714195
CCAATCC106900.010.4383597
AATCCCC108450.010.4219359
GTTTTAC121500.09.905641
ATCACAC67250.09.5274048
TTACCAA120500.09.1444464
CAATCCC126700.09.0343588
TCACACC77600.08.6277899
TTTTACC147900.07.8885732
TGAATCA91800.07.84215835
AATCACA91300.07.72740277
GAATCAC88050.07.3585366
TTTACCA153600.07.22075373
CTACCTA35150.06.8411183120-124
CCACTAA35850.06.30580320-24
ACCTAAG37600.06.2421727120-124
ATGAATC113250.05.97781754
CCCACTA41250.05.86422515-19
CTAAGTA45450.05.829347120-124
Produced by FastQC (version 0.11.5)