FastQCFastQC Report
Sat 11 May 2024
EGAF00005180766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13781001
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC1530911.1108844705838132No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG864890.6275959199190247No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA599570.43506999237573524No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA384120.27873156674177735No Hit
ATACAAACACTATAGCTCTCAAAATTTAAAGTACTAAATCCTTGAGGCCC260170.18878889857130118No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT178960.12985994268486012No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGGCATAATCTCGTAT153470.11136346336525192TruSeq Adapter, Index 20 (97% over 38bp)
GTAGCCCTTATTTAGAATAAGTCAAAAAAAAAGTAAGGGACAAATATTCC139660.10134242062677451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)