FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11825551
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACATCTCGTAT338800.28649827817748197TruSeq Adapter, Index 16 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA182100.034.670249
AGAGCAC198800.031.68438
GATCGGA223250.028.9071391
TCGGAAG242850.026.5267283
ATCGGAA260650.024.9923922
AAGAGCA256600.024.7997937
GGAAGAG315950.020.4143985
GAAGAGC331600.019.3209276
CGGAAGA347200.018.6395024
CTCGTAT112400.010.87744940-44
CAGTCAC125600.010.33075925-29
AGTCACC123200.010.27484325-29
TCGTATG120500.09.9426545-49
CCAGTCA133150.09.86394625-29
TCTCGTA126450.09.82828240-44
CACACGT146550.08.94984910-14
CACCCGA143000.08.94226530-34
ACCCGAC145900.08.77439330-34
CACGTCT152800.08.63088410-14
GTCTTCT155700.08.51721450-54
Produced by FastQC (version 0.11.5)