FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11825551
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTAACTCGTGTAGATCT364200.30797719277520347Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA223550.028.434381
AGAGCGT241050.026.2492268
TCGGAAG243000.026.2223113
ATCGGAA264650.024.1343292
AAGAGCG279550.022.8658967
GAGCGTC275550.022.6231089
GGAAGAG316200.020.1708015
GAAGAGC327750.019.481946
CGGAAGA343150.018.6950934
TAACTCG69350.017.52023135-39
GCTAACT72500.017.25622430-34
CTAACTC72050.017.12422230-34
GTGCTAA75100.016.65880630-34
TGCTAAC74850.016.59904130-34
ACTCGTG85100.014.86973235-39
TGTGCTA85700.014.49752930-34
CTCGTGT89000.014.21813635-39
AGTGTGC97750.013.09366125-29
GAGTGTG100950.012.57877625-29
TAGATCT109150.011.84554840-44
Produced by FastQC (version 0.11.5)