FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16645747
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC891060.5353079077796868No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG356030.21388646601441197No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTAT264010.15860507792170578TruSeq Adapter, Index 1 (97% over 37bp)
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA194520.11685867867630093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA227000.019.2503389
AGAGCAC232450.018.7364378
GATCGGA265350.017.038531
TCGGAAG275000.016.1419353
ATCGGAA308750.014.9609322
AAGAGCA307250.014.362497
GGAAGAG380400.012.016995
GAAGAGC390300.011.6384016
CGGAAGA401650.011.2378924
CTCGTAT107450.08.42836340-44
AGTCACA107700.08.42267825-29
TCGTATG124850.07.311320345-49
CAGTCAC129500.07.07151925-29
TCTCGTA131500.07.018289640-44
ACCAATC131650.06.89035235-39
CCAGTCA135450.06.86718725-29
CCAATCT131600.06.86014635-39
TCACAAC135950.06.714601530-34
CAACCAA138950.06.64234135-39
AACCAAT139750.06.563104635-39
Produced by FastQC (version 0.11.5)