FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18306618
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG174900.012.592989
TAGTGTA34400.011.09225756
GTAGTGT41800.09.3007945
TGTGTAG41900.08.7637882
CTCGTGT61600.08.6487535
AGTGTAC52250.07.99166157
TGTCTCG68150.07.81810672
GTGTACG122500.07.64016348
TCTCGTG82900.07.20818764
TGTAGTG56600.07.12318334
CTGTGTA64300.06.3992071
CTCTGTA75450.06.2977766
GTCTCGT88900.06.0743363
CTGTCTC99500.05.8338211
CTGTACG196750.05.5985058
TTGTACG89700.05.53798878
TCGTGTA109350.05.33296446
GTGTAGT76050.05.2071823
GTACGCT100450.05.16085773
CGTGTAC122250.05.0645987
Produced by FastQC (version 0.11.5)