FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14225589
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATG22700.013.9537388
CCTTTAG40100.07.36043074
AGTGAGT24153.5688572E-96.92454531
CCTAGAC5809.822543E-116.702467120-124
TTAGCAT53900.06.2772997
TTTAGCA60700.05.5740766
CTTTAGC60200.05.38120755
GTGAGTA27351.2652867E-55.270382
CCACTCA30753.4126751E-65.15114649
Produced by FastQC (version 0.11.5)