FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11470796
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACCAAATCTCGTAT132020.11509227432865166TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAT26900.065.045224
TGGCTAA33350.052.2568663
CTAATAC36900.047.019936
GCTAATA42950.041.0699845
TTGGCTA44850.039.660522
TAATACG48200.036.594047
ATTGGCT57550.031.8025171
AGTGAGT17950.022.9718881
ATACGAC81600.021.7920519
GAGCACA137350.020.337359
GGCTAGT16550.020.09738285-89
AGAGCAC138600.019.8422748
CTAGTCC16550.019.74937485-89
GATCGGA142850.019.4969541
AGGCTAG16500.019.37289485-89
GAGCTAG16650.019.02638460-64
GTGAGTA22700.018.4003472
TATCTTG92950.018.3565169
TATATCT96100.017.455167
TCGGAAG161500.017.0785143
Produced by FastQC (version 0.11.5)