FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11470796
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG174640.1522474987786375Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA24700.072.5686348
TCACTAA34900.052.1849639
TTAACCC35150.051.6220662
ATTAACC35450.050.9317321
TAACCCT38700.046.867293
CCTCACT45250.039.9302187
AACCCTC47150.039.3838584
GACTCGG48950.033.965623
ACCCTCA63050.030.1370685
CCCTCAC68550.027.5090756
ACTCGGT59850.027.4189594
CGACTCG60150.027.17382
CCGACTC62600.025.9111981
GATCGGA155750.024.1745781
GTGCCAC70500.023.2807039
TCGGAAG177400.021.827753
CTAGCCT16050.020.72160135-39
ATCGGAA189850.020.4427032
AGAGCGT197600.019.3443498
GGTGCCA84600.019.3153258
Produced by FastQC (version 0.11.5)