FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14438855
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGC10650.040.4811441
CTAACGC23050.022.191031
CTAGGGC10800.019.6211571
CTATCGC89350.017.501271
CTAGAGC15450.014.66167451
CTAGGGG5901.2481596E-613.6235731
TATCGCT117600.010.712772
CTATAGC39900.010.2557051
CTAGGTC8053.96553E-59.984981
CTAGCGA7401.8693626E-49.874581
CTAGTGC11903.9575752E-89.8247931
CTAGCCC10709.722553E-79.5608081
GGGCTAG9201.9493957E-59.3920584
CTAGGCC10155.1356037E-69.3589621
CTAGATC11602.9110652E-68.8190211
CTAGCTC10054.6420886E-58.7250021
CTAGGTG8406.654887E-48.6990351
CTAGGGA8406.654887E-48.6990351
CCGCTAG12358.247087E-68.1625994
CTAGGCT9150.00179444397.86975577
Produced by FastQC (version 0.11.5)