FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14457490
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAG3901.3262252E-820.3050692
CCTAGGT2750.00276074615.7101054
GCCCTAG4752.3156863E-615.1559352
CCCTAGT4051.5369563E-414.2213193
CCCTAGC5659.057585E-714.0168093
ACCTAGC4550.005907821511.076223
TCTAGAC4650.006937850710.8394084
GCTAGAG8558.193594E-610.1039582
GCTCTAG8559.0321264E-59.2619612
GCTAGTT6350.00660801179.0714514
GTTCTAG6350.0066185339.0696942
CCTAGCT6500.0080030498.862114
ACTAGGA7600.00297958968.5268655
AGCTAGA9450.00213367.73244861
TTCCTAG11400.00198456676.94647072
TCTAGTT10550.0072048846.8250844
GCTTCAG203550.06.7198392
GAGTTAG28454.3655746E-106.57911442
AGCTTCA195600.06.53759531
GCTTTAG72450.06.26006032
Produced by FastQC (version 0.11.5)