FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14195079
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTAT1605801.13123709984284TruSeq Adapter, Index 1 (97% over 39bp)
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC157990.11129913401679555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA367200.071.9536069
GATCGGA370300.071.891671
AGAGCAC376950.070.054288
TCGGAAG385400.069.218853
ATCGGAA422100.063.257272
AAGAGCA444750.059.9567457
GAAGAGC446250.059.8679286
GGAAGAG468400.057.4057275
CGGAAGA484050.055.26854
CTCGTAT259800.020.33420240-44
AGTCACT274250.020.28109625-29
TCTCGTA281550.018.98326740-44
CAGTCAC290450.018.89212825-29
GTCACTT299600.018.5938925-29
TCGTATG276850.018.46270445-49
CCAGTCA297850.018.4227625-29
ACGCAAT298850.018.37591635-39
CGTATGC311200.017.82207145-49
CACGCAA309300.017.72713935-39
ACACGTC314050.017.56435410-14
Produced by FastQC (version 0.11.5)