FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18285799
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTAT1974021.0795371862066294TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA459850.070.979059
AGAGCAC469000.069.591428
GATCGGA478450.068.767371
TCGGAAG527850.062.4880263
ATCGGAA542650.060.8365062
AAGAGCA561350.058.424777
GAAGAGC638350.051.5352486
CGGAAGA662500.049.9485784
GGAAGAG681550.048.7229965
CTCGTAT315700.021.0353640-44
AGTCACA335450.020.26664725-29
TCTCGTA329300.020.21033140-44
GTCACAA346200.019.56663925-29
TCGTATG327550.019.43593845-49
CAGTCAC353150.019.10818525-29
CACACGT360900.018.57057410-14
CCAGTCA364550.018.50669525-29
CACGTCT370300.018.4724210-14
CACAATC370350.018.18811230-34
ACACGTC380350.017.9540410-14
Produced by FastQC (version 0.11.5)