FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16914303
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG108500.025.5907421
AGACGGA213500.013.116832
TAGACTG38850.012.225071
TAGACGT50600.010.9746861
TAGACGC58400.09.7591221
TAGAGGG57000.09.1014911
AGACGTA66750.08.9516682
TAGACGA83250.07.89927631
TAGACTC22559.458745E-117.7766711
TAGAAGG74300.07.37566951
TAGACTT53850.06.5130521
TAGCCGG112950.06.4690841
AGAGGGA95000.06.44128372
TAGACTA24001.4655416E-76.3934761
AGACTGA78400.06.3359272
TAGATGG66250.06.2866311
TAGGCGG94500.05.7990721
AGACGCA108900.05.75133282
AGACTAA34405.653419E-95.6504422
GACGGAT199200.05.4576683
Produced by FastQC (version 0.11.5)