FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13044926
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG148590.11390635715373164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCC10950.030.024131
CTAGCCG12100.025.5829472
CTTAGCC37900.016.5779691
CATAGCC161350.015.7573191
TTAGCCG39200.015.2426162
ATAGCCG181650.014.0689092
TAGCCGG103900.012.0569133
CCTAGGC7109.9200315E-611.3189561
TAGCCGC93750.011.1352293
CCTAGTC7251.2510809E-511.0847721
CCTAGCG6757.386731E-510.8235151
CCTAGAC6153.7616017E-410.6915211
TAGCCGT95850.09.689473
ACTAGCC10054.538255E-69.4503811
CCTAGAA12306.42761E-68.3156271
TAGCCGA105200.08.1439233
CTTAGGC21402.5465852E-107.8521051
CTAGCGG9400.00234491597.6584282
CCTAGCA8600.0079486357.645681
TTAGTCG17502.2647746E-66.9932392
Produced by FastQC (version 0.11.5)