FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005180999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005180999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15798208
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT48050.028.1343571
GTGAGTA51500.025.5816252
TAACCCC58650.023.9409649
GTCTCTT78800.018.4537871
ATAACCC86350.017.5118478
TGAGTAT127000.010.8277263
GAGTATA133750.010.5519274
GTATAAC140850.010.42906956
CTCTTTA158200.010.1030883
TAGCATC170700.09.8709138
TATAACC162500.09.2612727
TTAGCAT180350.08.7439117
CTTTAGC216200.07.59366465
TTTAGCA239500.07.2758226
AACCCTA49750.07.206413785-89
TCTTTAG245250.06.7235094
ACCCTAT56700.06.34848185-89
CGCCTAA49300.06.33770945-49
TTAACCC50250.06.303773480-84
TCTCTTT264650.06.2294242
Produced by FastQC (version 0.11.5)