FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13644156
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAG10155.7940815E-79.9300162
GCGCTAG9051.608314E-59.5459822
GCTCTAG13855.4496923E-99.3564412
CCCTAGT8458.181597E-48.5202113
GCTTTAG45700.07.8766532
AGCTTCA189100.06.91692731
GCTTCAG174550.06.72288472
GCATTAG47100.05.96117162
GCTACAG102750.05.67532972
AGCTACA104500.05.4541871
GCCTCAG96850.05.12905552
GGCCTTA34658.9031164E-75.06127261
Produced by FastQC (version 0.11.5)