FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12537485
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA56750.026.130875
CAGTGGT59800.024.7984054
AGCAGTG86300.017.302692
GTGGTAT86050.016.8986236
GTATCAA98700.015.02722559
GGTATCA99250.014.9439528
GCAGTGG101300.014.7815023
TGGTATC102150.014.5901837
AAGCAGT117600.013.4868581
TCTTGGG65550.05.030026435-39
Produced by FastQC (version 0.11.5)