FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15655338
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACTC38250.042.9103432
GCTAACT39050.040.9754751
CTAACAC23300.033.985632
CTAACCC25500.032.465062
GCTAACA29600.029.1266961
GCTAACC29250.027.726691
TAACTCG33150.024.5400643
CTAACGC28400.019.7712822
GCTAACG33850.018.7785261
CCTAACT20200.015.9148391
ACTAACT25700.015.6361731
CTAACTG20750.015.2648712
GCTAAGT21850.014.3786441
GCTAATT37800.013.9168721
CTAGCTC9405.456968E-1213.7848872
CTCACTC40800.013.7623642
TAACTCA38100.013.6046793
CTAACTA18300.012.9813982
CTAACGT16700.012.5008662
TAACTCT48950.012.2069223
Produced by FastQC (version 0.11.5)