FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14360930
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT68850.030.3221234
AGCAGTG104350.020.2520332
AGTGGTA103600.020.220685
GCAGTGG121800.017.2584823
GTGGTAT121000.017.2534186
AAGCAGT158350.013.6721241
CCACTCA56900.012.90652759
TGGTATC170550.012.5379427
GTATCAA193150.011.7418729
GGTATCA193100.011.2974878
GCCACTC73050.010.5459358
AGCCACT94600.08.067457
AAGCCAC99850.07.57013756
CCGTTAA56550.05.8558614
CGTTAAA58650.05.64614875
TACAAAG159250.05.570822
ACAAAGC146350.05.50924873
CTCACTA29350.05.44532415-19
ATACAAA179750.05.31726031
Produced by FastQC (version 0.11.5)