FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14106812
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTAT197140.13974808766147873TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG68450.039.1266677
GGTCATA107000.025.4367872
GTCATAA109850.024.8423883
ATACGAC110800.024.1717539
GAGCACA205800.023.228859
GATCGGA210350.022.712671
AGAGCAC214050.022.5016468
GCTAGTC20850.021.61669285-89
ATAATAC131650.020.7813536
GAGCTAG21350.020.70540660-64
CATAATA132850.020.7021755
GGCTAGT22850.019.78765985-89
CTAGTCC22600.019.68797385-89
AGGCTAG24050.018.80033385-89
TCGGAAG260650.018.6190013
AGCTAGA24000.018.53917960-64
TATATCT130250.018.5169967
GCTAGAA25850.018.10364760-64
CATCTAG14000.017.9989645-49
ATCGGAA274100.017.7316422
Produced by FastQC (version 0.11.5)