FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14106812
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG278500.1974223516978889Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA45150.061.0481648
TTAACCC48200.058.9977762
TAACCCT59150.047.9392853
TCACTAA59850.046.0533949
ATTAACC65150.042.5990331
AACCCTC75550.038.390234
CCTCACT78950.035.093977
GACTCGG68150.035.0608373
ACCCTCA92250.031.8263555
ACTCGGT78650.030.6546364
CCCTCAC101650.028.0347276
CCGACTC90450.026.5546261
CGACTCG95250.025.3200992
GATCGGA225000.024.9950281
GTGCCAC108600.021.8018369
GGTGCCA110300.021.2050578
AGAGCGT274550.020.8652328
TCGGAAG278300.020.4297923
CTAGCTC24250.019.94841260-64
GACTAGC24050.019.93225130-34
Produced by FastQC (version 0.11.5)