FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15333948
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC521900.3403559213843688No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG326390.21285451078874143No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA200570.13080127831397367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT110650.041.4424364
AGTGGTA120300.037.8783535
AGCAGTG154500.030.1097982
GTGGTAT156100.029.4680236
GCAGTGG165600.028.2129653
TGGTATC179050.026.0152687
GGTATCA197350.024.2230768
GTATCAA200800.024.0220159
AAGCAGT201600.022.9848671
TCTTGGG112450.08.41231135-39
GCAGAGT137750.07.661730315-19
CAACGCA140450.07.586345710-14
CTAGGGG6200.07.432904795-99
ACTAGGG7500.07.296492690-94
AACGCAG156800.06.70346710-14
TCAACGC172700.06.203021510-14
ACGCAGA176800.06.14864115-19
TATCAAC182950.06.03650610-14
CGCAGAG183500.05.93198715-19
AGGGGGG36701.05501385E-105.9184471
Produced by FastQC (version 0.11.5)