FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15333948
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG473130.3085506746207826No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA314230.20492439390038367No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA221010.1441311787414435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA121050.032.8496327
CTCAAAC128750.031.4990779
TGGCTCA132500.030.1737376
ATGGCTC130450.029.8205955
TCTGATG162750.023.5366041
GCTCAAA181450.022.3505448
GATGGCT178300.022.34194
CTGATGG238450.016.7971882
TGATGGC263400.015.7514933
CCTCTAG16000.015.64097250-54
CTCGGGT59100.013.40786120-24
CTCTAGG11650.011.97513250-54
GGTCCTC85000.09.61134145-49
AGGTCCT88950.09.13602145-49
CTCTAGC12500.08.74456150-54
TCGGGTG99350.08.52614525-29
CTAGGAT17150.08.38629950-54
CTAGCAC12650.08.29978550-54
GGGTGGG102000.08.22003625-29
AACACAG112400.07.90705410-14
Produced by FastQC (version 0.11.5)