FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15067489
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCC11000.028.558591
CTAGCCG9950.026.0463242
CCTAGGC4501.200533E-1021.1053161
CTTAGCC34900.016.955891
CCTAGTC3800.001372915113.4578411
CTAGGCC3950.002052818412.757572
CTAGCCC6906.2372783E-712.5198512
CTAGGCG5259.9178345E-512.3409962
CATAGCC188250.012.069421
CCTAGCT5701.8605517E-411.5352941
TTAGCCG37000.011.2847842
CCTAGCA6505.032916E-511.2395161
CTAGCCT8104.299278E-610.6650572
ATAGCCG214700.09.321372
CCTAGCG7100.00140718629.2607291
CTAGCGG8206.074154E-48.7791642
CCTAACC20401.0913936E-118.5949241
TAGCCGG118500.08.5055273
CTTAGGC19702.7421265E-87.41693971
TAGCCGC118200.07.36986263
Produced by FastQC (version 0.11.5)