FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17048564
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTAT2480441.4549260571154263TruSeq Adapter, Index 16 (97% over 37bp)
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC249210.1461765342817143No Hit
ATTGGCTAATACGACTCACTATAGCCGTTTCAGGCTTAATTAAACCATCT190230.11158124520047553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATA155000.0104.096265
CTAATAC155700.0103.805626
TAATACG172800.092.741597
GGCTAAT182550.087.8434144
TTGGCTA197350.082.1767962
TGGCTAA201850.079.773933
ATACGAC204900.078.5287559
TATCTTG297950.076.113259
ATTGGCT223800.072.8898541
GCTTTAT314750.072.213941
ATATATC316150.071.845456
CTTTATA321300.070.602122
TATATCT326450.069.777117
AATACGA242250.066.54018
TTATATA392900.058.0962454
ATATCTT419550.054.7050868
TATATAT455500.050.154125
TTTATAT456350.050.071433
GAGCACA946200.047.1815229
AGAGCAC954250.046.7985888
Produced by FastQC (version 0.11.5)