FastQCFastQC Report
Sat 11 May 2024
EGAF00005181069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17048564
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2754441.6156434055091091Illumina Single End PCR Primer 1 (100% over 50bp)
CCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAA1407460.8255592670444267No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG275320.1614916071523678No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA196170.11506540961455758No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTAACTCGTGTAGATCT187820.11016763640620993Illumina Single End Sequencing Primer (100% over 32bp)
ATTAACCCTCACTAAAGGGATGACTGAATTCTTTGAAAAATGTGATCAAG187770.1101383084229264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)