FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13183008
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG181830.1379275503739359Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA37900.056.220088
TTAACCC51700.042.1978072
TCACTAA52700.040.978359
TAACCCT56250.038.1297073
CCTCACT60350.035.783517
ACCCTCA71000.031.7290695
ATTAACC71900.031.6574461
AACCCTC72300.030.9593494
CCCTCAC73550.029.4547396
GACTCGG59300.021.3613013
GATCGGA211950.020.231241
ACTCGGT64550.019.2894364
AGAGCGT220500.018.640888
TCGGAAG240000.018.293143
GTGCCAC70500.017.358169
ATCGGAA266100.016.6405642
AAGAGCG267150.016.3019337
GAGCGTC250400.016.1851949
GGAAGAG309300.015.4976445
CCGACTC82550.015.1208061
Produced by FastQC (version 0.11.5)