FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15980274
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA79750.046.4898535
CAGTGGT88400.042.760534
GTGGTAT113100.033.4166876
GTATCAA116750.032.1253249
AGCAGTG120200.031.6269512
GGTATCA127000.029.8159548
TGGTATC127150.029.3844597
GCAGTGG146500.025.7531093
AAGCAGT155600.024.7359031
TCTTGGG88550.08.68341835-39
GCAGAGT113750.07.265269815-19
CAACGCA131350.06.53289710-14
TATCAAC130750.06.50781810-14
ACCCTAG3754.633043E-56.144088380-84
CCCTAGT4257.0263395E-66.098853685-89
CTTGGGA74400.05.24486635-39
AACGCAG175200.05.160777610-14
Produced by FastQC (version 0.11.5)