FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15980274
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC110550.027.6738325
TGGCTCA114300.027.0163756
GATGGCT132550.023.0812424
GGCTCAA138350.022.8403477
CTCAAAC143150.022.8317389
TCTGATG136800.022.3662681
GCTCAAA197900.016.2605868
CCTCTAG13700.015.55496250-54
CTCTAGG8450.012.78004550-54
CTGATGG248350.012.75735952
TGATGGC270800.011.8826763
TCTAGGA9550.011.4587750-54
CTAGCAC10150.010.781404550-54
CTAGGAT10400.010.52223650-54
TCTAGCA13450.08.45731650-54
CTCTAGC12900.08.37142450-54
CTCGGGT79850.08.36670620-24
AACACAG85600.08.20846910-14
GGTGGGA92100.07.269203725-29
ACACAGC99400.07.097833610-14
Produced by FastQC (version 0.11.5)