FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11026369
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA94150.028.0581577
ATGGCTC119150.021.9304525
TGGCTCA123800.021.338256
CTCAAAC134350.019.9335969
GCTCAAA154150.017.46668
GATGGCT164900.016.0645334
TCTGATG171900.015.079391
CTGATGG216050.012.3650682
TGATGGC235200.011.8444963
CTCGGGT45400.011.67084620-24
CCTCTAG14650.010.81129350-54
GGGTGGG65600.08.29618125-29
GGTCCTC66400.08.17455145-49
GGTGGGA69250.08.00444425-29
AGGTCCT70700.07.982837745-49
ACACAGC75150.07.740521410-14
TCGGGTG71150.07.649043625-29
AACACAG79500.07.280760310-14
CAGGTCC82100.07.11989445-49
TGGGAAC81450.07.05381530-34
Produced by FastQC (version 0.11.5)