FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19872338
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT119850.041.2107624
AGTGGTA122400.040.523885
GTGGTAT154850.032.542696
AGCAGTG161200.031.1301612
GTATCAA162950.030.7041519
TGGTATC172350.029.071317
GGTATCA175250.028.6724018
AAGCAGT194050.026.2568261
GCAGTGG201400.024.7741283
GCAGAGT135900.07.765553515-19
TCTTGGG133700.07.722152735-39
CAACGCA165150.06.82605110-14
TATCAAC165450.06.770164510-14
AACGCAG189650.05.951817510-14
TCAACGC238350.05.13440510-14
Produced by FastQC (version 0.11.5)