FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19872338
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC153700.028.7127159
TGGCTCA154950.027.7332296
ATGGCTC161850.026.2410415
TCTGATG163600.026.0909121
GGCTCAA170950.025.5165737
GCTCAAA207600.021.7780978
GATGGCT200800.021.1155344
CCTCTAG17900.014.96148650-54
CTGATGG307650.014.1838492
TGATGGC362700.012.2459193
CTAGCAC12650.011.3821550-54
CTCTAGG12500.011.05798550-54
TCTAGGA14350.09.83306750-54
AACACAG104150.09.26276410-14
CTCTAGC15100.09.24931550-54
CTAGGAT14900.09.08356650-54
ACACAGC125700.07.86948910-14
TCTAGCA17900.07.802495550-54
CTCGGGT118550.07.651534620-24
GGGTGGG129850.07.229313425-29
Produced by FastQC (version 0.11.5)