FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16180917
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAG7100.021.60591
GCCTAGC9050.021.4782922
AGCGTAG90500.017.7576521
CCTAGCG7850.016.5083473
CCTAGCA9600.014.9989013
GACTAGC7104.0417945E-914.1956182
GCGTAGC121000.013.0894652
CCTAGCT6353.3649849E-612.4715283
TCCTAGC8555.4396878E-811.7881732
CTAGCCG9653.2546777E-69.7002714
GGCTAGC8508.4704734E-59.316622
ACCTAGC6350.00661757459.0698662
CTAGCGG9753.8648148E-58.8622594
GCGTAAC63100.08.55692
CGTAGCA61850.08.2645523
GCTTAGC31300.08.050232
CGTAGCT71200.07.98817973
AGCGTAA86550.07.68043381
AGCTTAG26951.8189894E-127.58945661
GCCTAAC17352.489851E-77.4689172
Produced by FastQC (version 0.11.5)