FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13548437
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCG8750.022.2148042
CCTAGCC9500.016.148221
CCTAGTC5104.1614803E-614.3238181
CATAGCC180450.011.3757071
ATAGCCG194600.010.1736472
CCTAGCG5800.00277733910.0760651
CTAGGCG7954.0706866E-59.9612172
GCTAGCC7351.7507745E-49.9389761
TAGCCGG93000.09.9091473
CCTAGCA7401.8746158E-49.871821
CCTAGGC7452.00621E-49.8055681
CTTAGCC39950.08.5942911
ATGCTAG7850.00399565148.2543343
CTAAGCC25200.08.1168311
TTAGCCG39650.07.44436172
TAGCCGC105300.07.2474753
CTTAGTC18504.2998927E-66.7128381
TAGCCGA106650.06.54817253
CATACCC95300.06.43895441
TTAGCCC20853.4199726E-55.86988542
Produced by FastQC (version 0.11.5)