FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18999226
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC141850.08.6824042
ACGCCCC149800.08.3598263
GATCGGA177350.07.89160971
CCCCGCG168350.07.1816276
TCGGAAG216700.07.10746433
GCTGACA156800.06.6557979
CTACGCC194350.06.5296961
ATCGGAA266300.05.8148762
AGAGCGT271650.05.7228598
AGCTGAC193500.05.43051348
GAGCGTC297100.05.2811929
GCCCCGC262600.05.12476065
Produced by FastQC (version 0.11.5)