FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9964903
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA89150.024.2217777
CTCAAAC94350.023.3486589
TGGCTCA112950.019.8826586
ATGGCTC112700.019.4806675
GCTCAAA113300.019.1893148
TCTGATG147250.014.7243351
GATGGCT166200.013.6432664
CTGATGG193050.011.5634572
CCTCTAG12200.011.09430250-54
CTCGGGT45650.010.06124720-24
TGATGGC237200.09.9845053
GGGTGGG54600.08.41161625-29
CTCTAGG9550.07.689512750-54
GGTCCTC62650.07.307982445-49
ACACAGC66050.07.259306410-14
AACACAG69050.06.881355310-14
AGGTCCT68600.06.758078645-49
GGTGGGA68600.06.63199825-29
CAAACAC75900.06.468987510-14
ACACGTT46650.06.451149535-39
Produced by FastQC (version 0.11.5)