FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15697616
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG704090.4485330766149459No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA538340.3429437947775No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC421710.2686458886495886No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA418440.2665627697861892No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC290670.18516824465574902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC180550.066.628925
GGCTCAA186850.065.037447
CTCAAAC207400.058.7656979
GATGGCT222550.054.4421464
TCTGATG230800.052.6177061
TGGCTCA231500.052.4003226
GCTCAAA253400.048.239928
CTGATGG328550.036.9885672
TGATGGC364250.033.7171823
CCTCTAG36450.021.3861250-54
CTCGGGT119850.019.52287720-24
CTCTAGG25500.019.51489450-54
TCTAGGA31650.015.76834250-54
TCGGGTG150400.015.67240725-29
CTAGGAT32850.015.49880350-54
AACACAG171950.014.82861510-14
TGGGAAC161600.014.6038430-34
AGGTCCT164400.014.47914145-49
GGTCCTC167800.014.34004745-49
AACACGT96600.014.27714430-34
Produced by FastQC (version 0.11.5)