FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13702421
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA103300.027.9426447
CTCAAAC119650.024.1875729
ATGGCTC119400.023.7539545
TGGCTCA124700.022.801016
TCTGATG141400.019.8854121
GCTCAAA158950.018.4337128
GATGGCT155600.018.274184
CTGATGG238700.012.035992
CCTCTAG14450.011.25986450-54
TGATGGC278650.010.64363
CTCGGGT56100.010.21470720-24
CTAGCAC9650.09.25096250-54
CTCTAGG11050.07.687980750-54
AACACAG83750.07.392590510-14
TCGGGTG90700.06.74635225-29
TCTAGCA13700.06.72638950-54
GGGTGGG95750.06.405575825-29
CTCTAGC14450.06.27762350-54
CTAGGAT13150.06.241250550-54
TGGGAAC99900.06.169103630-34
Produced by FastQC (version 0.11.5)